Formed in 1974, Gallery 25 is one of the oldest cooperative Galleries in the country. Located in downtown Fresno, the gallery has been a forum for contemporary art since its inception.

The gallery was founded by Joyce Aiken, professor of art at California State University, Fresno. Professor Aiken was the director of the second feminist art program (the first program was created by Judy Chicago at CSUF in 1970). The program focused on women creating artwork from their experience as women. By establishing the gallery, Aiken gave the women in the program the chance to exhibit their work to the public and gain professional experience as artists.

The 25 founding members began exhibiting at 1986 Echo Street , later moving to a larger space at 1526 Fulton Street in 1981. The Gallery opened its doors to men as well as women in 1989. To expand its quarters in April of 2004, the gallery moved to its present location, 660 Van Ness, adjacent to several other galleries and artist studios.

Gallery 25 is a non profit organization dedicated to educating the public. Seminars, discussion groups and classes are held in relation to exhibits. The gallery also participates in international exhibitions and gallery exchanges.

The cooperative nature of Gallery 25 requires all members to share responsibility for its function including exhibit installation, maintenance of the gallery space, publicity and fundraising. Member's dues and the sale of artwork support ongoing operations of the gallery. Artists wishing to join the gallery are encouraged to submit a professional portfolio of visual work and a biography to be reviewed by the membership.

The exhibition schedule includes alternating monthly solo, dual and three person shows. Group exhibits are held several times a year and may include invited guest artists. Gallery exhibit information is featured weekly in the Sunday edition of the Fresno Bee Gallery Calendar, ArtScene Monthly Digest and Artweek Magazine.